A DOT physical urine test screens for medical conditions, drug use, and hydration levels to ensure commercial drivers meet safety standards. It checks for sugar, protein, blood, and drug metabolites, which may indicate diabetes, kidney disease, or substance use. Employers and the Department of Transportation (DOT) require this test for driver certification.
Understanding the DOT Physical Urine Test
Over 3.5 million commercial drivers in the U.S. must pass a DOT physical to legally operate a vehicle. One crucial component is the urine test, which helps determine if a driver is physically capable of safely performing their job.
A standard DOT urinalysis includes two primary components: a health screening (detecting medical conditions) and a drug test (checking for illegal substances). While not all employers require a drug test as part of a DOT physical, the urinalysis for health screening is mandatory.
Why is a Urine Test Required for a DOT Physical
The DOT urine test is not just about drugs—it identifies medical conditions that could compromise driver safety. Here’s what it helps assess:
- Kidney function – Detects protein, blood, or abnormal substances.
- Diabetes risk – High glucose levels indicate potential diabetes.
- Infections – Bacteria or white blood cells may suggest a urinary tract infection.
- Liver function – Bilirubin in urine may indicate liver disease.
- Dehydration – Concentrated urine suggests poor hydration, which affects physical and cognitive performance.
What Substances Are Tested in a DOT Urinalysis?
A DOT physical urine test screens for several key indicators. Here’s a breakdown:
Glucose (Sugar)
- High glucose levels may indicate undiagnosed diabetes.
- Uncontrolled diabetes can lead to fatigue, dizziness, and poor reaction times.
Protein
- Excess protein in urine may signal kidney disease or high blood pressure.
- Drivers with kidney issues may require further medical clearance.
Blood
- The presence of red blood cells could indicate kidney stones, infections, or bladder issues.
- Even trace amounts require further testing.

Specific Gravity & Hydration Levels
- Measures how concentrated urine is, indicating hydration status.
- Dehydration can lead to fatigue, dizziness, and cognitive impairment, affecting driving ability.
Ketones
- Ketones in urine could suggest uncontrolled diabetes or extreme dieting.
- Can lead to dangerous conditions like diabetic ketoacidosis.
pH Levels
- The pH of urine can signal kidney problems or metabolic imbalances.
- Abnormal levels may require additional medical review.
Does a DOT Physical Test for Drugs?
Not all DOT physicals include a drug test, but many employers require it separately. If drug screening is included, the test will check for:
- Marijuana (THC)
- Cocaine
- Opiates (heroin, morphine, codeine)
- Amphetamines (meth, Adderall, ecstasy)
- Phencyclidine (PCP)
Failing a DOT drug test can lead to job disqualification and mandatory substance abuse treatment before reapplying for certification.
How to Prepare for Your DOT Urine Test
Proper preparation ensures accurate test results. Follow these tips:

What Happens If You Fail the Urine Test
A failed DOT urine test doesn’t always mean immediate disqualification. The medical examiner will:
- Request additional testing – If an abnormality appears, you may need a second urinalysis.
- Refer you for further evaluation – If diabetes, kidney issues, or infections are suspected, you may need clearance from a specialist.
- Provide temporary disqualification – In cases of drug test failure, you may need to complete a substance abuse program before reapplying.
Stay Certified with Our Reliable DOT Physical Exams
If you need a DOT physical, visit Centers Urgent Care for a quick and professional medical exam. Our experienced providers ensure that your urine test and other required screenings are done efficiently. We serve commercial drivers in urgent care in New York with fast, high-quality medical services. Contact us today to schedule your DOT physical!
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